Intermediate
Part:BBa_K300980:Design
Designed by: Susanna Zucca, Federica Sampietro, Paolo Magni Group: iGEM10_UNIPV-Pavia (2010-10-09)
BBa_K300001 construction intermediate (provided by Mr Gene)
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 72
Illegal XbaI site found at 134
Illegal SpeI site found at 90
Illegal PstI site found at 8
Illegal PstI site found at 349
Illegal PstI site found at 1695 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 72
Illegal NheI site found at 1637
Illegal NheI site found at 1688
Illegal SpeI site found at 90
Illegal PstI site found at 8
Illegal PstI site found at 349
Illegal PstI site found at 1695 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 72
Illegal BglII site found at 139
Illegal XhoI site found at 1587 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 72
Illegal XbaI site found at 134
Illegal SpeI site found at 90
Illegal PstI site found at 8
Illegal PstI site found at 349
Illegal PstI site found at 1695 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 72
Illegal XbaI site found at 134
Illegal SpeI site found at 90
Illegal PstI site found at 8
Illegal PstI site found at 349
Illegal PstI site found at 1695 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
See BBa_K300001.
Source
Mr Gene synthesis service (www.mrgene.com).
References
See BBa_K300001.