Intermediate

Part:BBa_K300980:Design

Designed by: Susanna Zucca, Federica Sampietro, Paolo Magni   Group: iGEM10_UNIPV-Pavia   (2010-10-09)

BBa_K300001 construction intermediate (provided by Mr Gene)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 72
    Illegal XbaI site found at 134
    Illegal SpeI site found at 90
    Illegal PstI site found at 8
    Illegal PstI site found at 349
    Illegal PstI site found at 1695
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 72
    Illegal NheI site found at 1637
    Illegal NheI site found at 1688
    Illegal SpeI site found at 90
    Illegal PstI site found at 8
    Illegal PstI site found at 349
    Illegal PstI site found at 1695
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 72
    Illegal BglII site found at 139
    Illegal XhoI site found at 1587
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 72
    Illegal XbaI site found at 134
    Illegal SpeI site found at 90
    Illegal PstI site found at 8
    Illegal PstI site found at 349
    Illegal PstI site found at 1695
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 72
    Illegal XbaI site found at 134
    Illegal SpeI site found at 90
    Illegal PstI site found at 8
    Illegal PstI site found at 349
    Illegal PstI site found at 1695
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

See BBa_K300001.


Source

Mr Gene synthesis service (www.mrgene.com).


References

See BBa_K300001.